炙甘草药材指纹图谱研究(3)
[12] Su Zhi Heng， Zou Guo An， Alfred Preiss， et al. Online identification of the antioxidant constituents of traditional Chinese medicine formula Chaihu-Shu-Gan-San by LC-LTQ-Orbitrap mass spectrometry and microplate spectrophotometer [J]. J Pharmaceut Biomed， 2010， 53(3)：454.
HPLC fingerprint of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle
SUN Lei， JIN Yong， LIU Xiao-qing， QIAO Shan-yi， GAO Song， CHE Yan-zhong
(1. Institute of Pharmacology and Toxicology， Academy of Military Medical Sciences， Beijing 100850， China；
2.School of Pharmaceutical College， Central South University， Changsha 410013， China；
3.Henan Wanxi Pharmaceutical Co.， Ltd.， Nanyang 473000， China)
[Abstract] The chromatographic fingerprint was established by eluting with the mobile phase consisted of acetonitrile and 0.2% formic acid water on an Agilent TC-C18(2) column (4.6 mm×250 mm， 5 μm). Six chromatographic peaks were identified by HPLC-MS/MS method. Ten batches of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle were determined， and the similarity was arranged from 0.72 to 0.99. Good precision， stability and repeatability were obtained， and this study provides a reference for the quality control of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle.
[Key words] Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle； HPLC； fingerprint； quality control
doi：10.4268/cjcmm20141122
[责任编辑 孔晶晶], 百拇医药(孙磊 靳勇 刘晓晴 乔善义 高松 车彦忠)
HPLC fingerprint of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle
SUN Lei， JIN Yong， LIU Xiao-qing， QIAO Shan-yi， GAO Song， CHE Yan-zhong
(1. Institute of Pharmacology and Toxicology， Academy of Military Medical Sciences， Beijing 100850， China；
2.School of Pharmaceutical College， Central South University， Changsha 410013， China；
3.Henan Wanxi Pharmaceutical Co.， Ltd.， Nanyang 473000， China)
[Abstract] The chromatographic fingerprint was established by eluting with the mobile phase consisted of acetonitrile and 0.2% formic acid water on an Agilent TC-C18(2) column (4.6 mm×250 mm， 5 μm). Six chromatographic peaks were identified by HPLC-MS/MS method. Ten batches of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle were determined， and the similarity was arranged from 0.72 to 0.99. Good precision， stability and repeatability were obtained， and this study provides a reference for the quality control of Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle.
[Key words] Glycyrrhizea Radix et Rhizoma Praeparata Cum Melle； HPLC； fingerprint； quality control
doi：10.4268/cjcmm20141122
[责任编辑 孔晶晶], 百拇医药(孙磊 靳勇 刘晓晴 乔善义 高松 车彦忠)