【摘要】 目的：对多重PCR和荧光PCR两种核酸检测方法在多项目质控样本中的检测应用进行初步评价。方法：同时采用三种方法对多项目盲样质控样本进行检测，包括多重PCR、荧光PCR及传统的国标方法。结果：食源性致病菌盲样(W-B11)中采用多重PCR、荧光PCR均检出金黄色葡萄球菌和蜡样芽孢杆菌核酸阳性，培养法同步分离并鉴定为金黄色葡萄球菌和蜡样芽孢杆菌，三种方法检测结果一致。结论：PCR法结合培养法对于多项目质控样本中病原菌快速初筛分离具有重要意义，可减少传统培养法漏检的可能性，提高检测效率。

    【关键词】 细菌盲样； 聚合酶链反应； 多项目质控考核

    【Abstract】 Objective：To evaluate multi-PCR and real-time PCR methods on detection of multi-blind quality control projects.Method：Multi-PCR，real-time PCR and traditional international standard method were used in the blind quality control projects at the same time.Result：Multi-PCR and real-time PCR methods detected W-B11 positive of Staplylcoccus aureus nucleic acid and Bacillus cereus nucleic acid by both.The culture method was isolated and identified as Staphylococcus aureus and Bacillus cereus.The results of the three methods were consistent.Conclusion：The PCR method combined with cultivation method for pathogenic bacteria in the multi projects quality control sample rapid screening has important significance in separation ......